| RQP74009 | |
| I. Background | |
| CD40, a TNF receptor superfamily member, was initially identified on B lymphocytes. However, antigen presenting cells (APCs) such as monocytes, basophils, dendritic cells and non-immune cells like endothelial cells and epithelial cells have been found to express CD40. A wide variety of carcinoma cells also over-express CD40. Interaction with CD40 ligand (CD40L, CD154) on CD4+ T helper lymphocytes triggers the expression of intercellular adhesion molecule (ICAM) and other pro-inflammatory cytokines. CD40:CD40L signaling simultaneously increases activation of antigen-specific T cells. CD40 also activates NF-κB-dependent signaling in response to lipopolysaccharide (LPS) found on Gram negative bacterial pathogens. Furthermore, agonistic CD40 monoclonal antibodies have been shown to activate antigen presenting cells (APC) and promote anti-tumor T-cell responses in addition to fostering cytotoxic myeloid cells with the potential to control cancer in the absence of T-cell immunity. | |
| II. Description | |
Recombinant HEK293 cell line expressing full length human CD40 (Tumor necrosis factor receptor superfamily member 5; TNFRSF5). This NF-ĸB luciferase reporter construct is stably integrated into the genome. | |
| III. Introduction | |
| Host Cell: | HEK293 |
| Expressed gene: | CD40 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | NF-κB cell line, NFkappaB cell line, luciferase reporter, NF-kB cell line, NFkB cell line, CD40 cell line, cd40 luciferase reporter, CD40 HEK293 cell line |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | MEM + 10% FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)+1ug/ml puromycin+100ug/ml hygromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: | kidney |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| Ⅴ. Representative Data | |
Figure 1. Recombinant HEK293 cell line expressing firefly luciferase gene under the control of NFκB response elements with constitutive expression of CD40. | |
Figure 2. Detect Luciferase assay by Ultra Luciferase Detection Kit RQPH0001（we strongly suggest to purchase from Reqbio). HEK293-CD40-NFκB Luciferase Reporter cells were stimulated by CD40L, the EC50 was 43ng/ml. | |
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