Service Background

Studying the function of genes in metabolic pathways or in the occurrence of certain diseases is of great significance for the study of disease mechanisms and drug development. The new-generation gene editing technology CRISPR-Cas9 has become a powerful tool for forward genetic screening experiments due to its unique advantages of simple operation and versatility. CRISPR guide sgRNAs are ideal for high-throughput screening of important molecular targets. Use CRISPR-Cas9 technology to establish a mutant library that may be related to a certain type of function, and confirm the genes related to this type of function through functional screening and enrichment, PCR amplification and deep sequencing analysis.

Reqbio provides customers with one-stop gene function screening services from sgRNA library design, chip Oligo primer synthesis, library construction and lentiviral packaging to screening.

Service Advantage

·The entire service process strictly abides by the QC standard to ensure that the storage capacity of the sgRNA plasmid library reaches more than 500 times the number of sgRNA; in addition, the sgRNA coverage of the library will be verified by NGS sequencing to reach more than 99%.

·he lentiviral transfection system is used to effectively improve the efficiency of sgRNA transfection.

·Rich screening platform: control cell survival, combined with immunostaining, flow cytometry and other screening methods.

·Provide customers with one-stop gene function screening services from sgRNA library design, chip Oligo primer synthesis, library construction and lentiviral packaging to screening.

Service Process

CRISPR-Cas9 sgRNA library construction service process

CRISPR-Cas9 sgRNA library technology roadmap

 Service Items

Ordering method

  Service Hotline：4008-750-250                                  Phone：180-6607-1954

  QQ：4008-750-250                                                     Mail：sales@cobioer.com