RNAi Technology Service

Studies in recent years have shown that introducing double-stranded RNA (dsRNA) composed of sense RNA and antisense RNA homologous to mRNA into cells can specifically degrade mRNA, thereby inhibiting the expression of its corresponding gene. This post-transcriptional gene silencing mechanism (Post-transcriptional gene silencing, PTGS) is called RNA interference (RNAi). Because RNAi has a high degree of sequence specificity, it can specifically silence specific genes, gain loss of function or reduce mutations, so RNAi can be used as a powerful research tool for the study of functional genomes.

Reqbio applies the RNAi Designer platform of a well-known foreign company, which can evaluate the interference position effect and sequence homology analysis of the target gene sequence, complete the design of various siRNA/miRNA sequences, and provide services from siRNA synthesis, RNAi vector construction to RNAi functional verification and other comprehensive RNAi solutions.

siRNA Synthesis Service

For transient gene silencing experiments, the method of chemically synthesizing siRNA is easy to operate, easy to obtain a high level of transient silencing effect, and has strong specificity. We enhance the specificity of siRNA through strict design; 3 siRNAs designed for a target gene can ensure that the knockdown efficiency of two of them reaches 75% (when the transfection efficiency is greater than 80%).

In the RNAi effect stage, the siRNA duplex binds a ribozyme complex to form the so-called RNA-induced silencing complex (RISC). Activated RISC localizes to cognate mRNA transcripts through base pairing and cleaves the mRNA at a position 12 bases from the 3' end of the siRNA.

siRNA Synthesis Experimental Process

1) Carry out chemical synthesis according to the sequence information;

2) Purification and quantification;

3) freeze-drying treatment;

4) Cell transfection pre-experiment, optimize transfection conditions;

5) Transient transfection or stable transfection;

6) RT-PCR or Western blot to detect changes in the RNA level or protein level of the target gene at a certain time point after transfection.

Quality Guarantee

Three siRNAs designed for a target gene can guarantee the Knockdown efficiency of two of them reaches 75% (when the transfection efficiency is greater than 80%).

shRNA Construction Services

Constructing shRNA expression vectors to achieve stable expression of shRNA in cells can make up for the short duration of transient transfection.。

shRNA Construction Experimental Process

1) mRNA sequence analysis;

2) Primer design and synthesis;

3) Annealing of single-stranded complementary primers;

4) Cloning the fragment into the vector;

5) Sequencing to ensure the correctness of the inserted sequence;

6) Cell transfection pre-experiment, optimize transfection conditions;

7) Transient transfection or stable transfection;

8) RT-PCR or Western blot to detect changes in the RNA level or protein level of the target gene at a certain time point after transfection.

Quality Guarantee

For the 3 shRNA sequences designed for a target gene, under the premise that the transfection efficiency is >80%, we guarantee that at least one clone can achieve a silencing efficiency of 70% transcription level.

miRNA Construction Services

The RNAi vector utilizes the endogenous miRNA processing mechanism of the cell, can express the RNA hairpin structure more efficiently than the traditional shRNA vector, and has the advantage of using GFP for expression tracking. We guarantee that at least 2 of the 4 sequences designed for each gene can achieve a knockdown efficiency of at least 70% transcription level (in the case of a transfection efficiency greater than 80%).

miRNA Construction Experimental Process

1) For a specific gene, design miR RNAi sequence, synthesize the sequence and anneal to generate double-stranded DNA;

2) Ligate the DNA with the vector and transform;

3) Sequencing to verify the correctness of the miRNA sequence;

4) Cell transfection pre-experiment, optimize transfection conditions;

5) Transient transfection or stable transfection;

6) RT-PCR or Western blot to detect changes in the RNA level or protein level of the target gene at a certain time point after transfection.

Quality Guarantee

For the 3 miRNA sequences designed for a target gene, under the premise that the transfection efficiency is >80%, we guarantee that at least one clone can achieve a silencing efficiency of 70% transcription level.

Customer Provided

1) Accession Number of the target gene (Human, Mouse or Rat species) and technical requirements (including the exact number of OD and nmols, purification type and modification requirements, etc.);

2) Cell lines and detailed cell culture procedures;

3) Primary antibody (if Western blot detection is required).

Service Description

1. RNAi technology services do not include gene function testing, if testing is required, additional assessment and charges are required;

2. Customers can provide experimental raw materials, or search for our company's template library, vector library, cell library, or purchase them by our company;

Ordering method

  Service Hotline：4008-750-250                                  Phone：180-6607-1954

  QQ：4008-750-250                                                     Mail：sales@reqbio.com