and uses directly on cells expressing firefly luciferase. This kit is designed to be used for the ultra-sensitive assay.

Component A:

1.Reqbio-UItra VS Bright-glo

Figure 2. TNF α Dose Response Curve.

Figure 3. NF-KB Hek293 cells.

2.freeze-thaw test

Figure 4. TNF α Dose Response Curve.

3.Quantification and Lysis Capacity Testing

Figure 5. Ultra vs Bright-glo.

1. Preparation of Assay Reagents

Dissolve the substrate and cell lysate at 4°C or room temperature before use. It is recommended to centrifuge before opening the cap to concentrate the liquid to the bottom of the bottle. Add component B to component A according to the volume ratio of 1:50, mix thoroughly to prepare the required amount of detection reagent.

2. Detection of Firefly Luciferase

• Equilibrate the detection reagent and test cell sample to room temperature.

• Add an equal volume of detection reagent to the test cell sample.

96-well plate detection system, add 100 μL detection reagent to 100 μL culture medium.

Shake at 1200 rpm for 5 minutes before measurement, full vibration will make the effect better.

3. Notes

·Reagents in kits with different batch numbers cannot be mixed.