| RQPC0002 | |
| I. Background | |
| Human Angiotensin converting enzyme 2 (ACE2), also known as ACEH, is an integral membrane protein found on the surface of cells in the lungs, arteries, heart, kidney, and intestines. | |
| II. Description | |
ACE2 serves as the entry point into cells for some coronaviruses, including the two strains that caused outbreaks of Severe acute respiratory syndrome (SARS-CoV) and coronavirus disease 2019 (COVID-19) (SARS-CoV-2). | |
| III. Introduction | |
| Host Cell: | HEK293 |
| Expressed gene: | ACE2 |
| Stability: | 32 passages |
| Synonym(s): | Angiotensin converting enzyme 2, ACEH, SARS-CoV, Coronavirus, COVID-19, SARS-CoV-2, ACE2 cells, ACE2 cell assay, covid19 |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+2ug/ml puromycin |
| Application(s): | Antibody Neutralizing Assay with 2019-nCov-S Pseudovirus |
| Storage: | Liquid nitrogen |
| IV. Description of Host Cell Line | |
| Organism: | Homo sapiens, human |
| Tissue: | Embryonic kidney |
| Disease: | Normal |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| Ⅴ. Representative Data | |
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Figure 1. Recombinant HEK293 cell constitutively expressing full length human ACE2 (Genbank NM_021804.3). | |
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