| RQP74031 | |
| I. Description | |
| NF-kB luciferase reporter construct is stably integrated into the genome of Jurkat T- cells. The firefly luciferase gene is controlled by 4 copies of NF-kB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-kB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene. | |
| II. Introduction | |
| Host Cell: | Jurkat |
| Expressed gene: | NF-kB Luciferase |
| Stability: | 32 passages |
| Synonym(s): | Nfkb, nf-kb reporter, nfkb report, nf-kb jurkat |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | 1640+10%FBS+200ug/ml hygromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | The NF-kB-luciferase/Jurkat cell line is suitable for monitoring the activity of NF-?B signaling in response to stimulants, and establishing cell-based screens for inhibitors that target specific NF- ?B-stimulating molecules. |
| III. Description of Host Cell Line | |
| Organism: | Homo sapiens, human |
| Tissue: | Peripheral blood |
| Disease: | Acute T cell leukemia |
| Morphology: | Lymphoblast |
| Growth Properties: | Suspension |
| IV. Representative Data | |
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Figure 1. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. Jurkat-NF-kB Luciferase Reporter cells were stimulated by Forsklin, the S/B was 12.43-fold. | |
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