| RQP74058 | |
| I. Background | |
| The transforming growth factor beta (TGFβ) signaling pathway is involved in a diverse range of cell processes such as differentiation, cell cycle arrest, and immune regulation. TGFβ signaling has been linked to cardiac disease, cancer, Alzheimer’s and other human diseases. TGFβ proteins bind to receptors on the cell surface, initiating a signaling cascade that leads to phosphorylation and activation of SMAD2 and SMAD3, which then form a complex with SMAD4. The SMAD complex then translocates to the nucleus and binds to the SMAD binding element (SBE) in the nucleus, leading to transcription and expression of TGFβ/SMAD responsive genes. | |
| II. Description | |
| The SBE Reporter -HEK293 Cell Line is designed for monitoring the activity of the TGF /SMAD signaling pathway. The transforming growth factor beta (TGF) signaling pathway is involved in a diverse range of cell processes such as differentiation, cell cycle arrest, and immune regulation. TGF signaling has been linked to cardiac disease, cancer, Alzheimer’s and other human diseases. TGF proteins bind to receptors on the cell surface, initiating a signaling cascade that leads to phosphorylation and activation of SMAD2 and SMAD3, which then form a complex with SMAD4. The SMAD complex then translocates to the nucleus and binds to the SMAD binding element (SBE) in the nucleus, leading to transcription and expression of TGF / SMAD responsive genes. | |
| III. Introduction | |
| Host Cell: | HEK293 |
| Expressed gene: | SBE-Luciferase |
| Stability: | 32 passages |
| Synonym(s): | SBE reporter, TGFβ reporter, TGFbeta reporter, SMAD reporter |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+100ug/ml hygromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | · Monitor TGF signaling pathway activity. · Screen for activators or inhibitors of the TGF/SMAD signaling pathway. |
| IV. Description of Host Cell Line | |
| Organism: | Homo sapiens, human |
| Tissue: | Embryonic kidney |
| Disease: | Normal |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| Ⅴ. Representative Data | |
| |
Figure . Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. HEK293/SBE Luciferase Reporter cells were stimulated by TGF, the S/B was 138.3-fold. | |
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