RQP30003L | |
I. Introduction | |
Host Cell: | SW480 |
Expressed gene: | Luciferase |
Stability: | 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Freeze Medium: | 90% FBS+10% DMSO |
Culture Medium: | DMEM+10%FBS+2ug/ml puromycin |
Mycoplasma Status: | Negative |
Storage: | Liquid nitrogen |
II. Description of Host Cell Line | |
Organism: | Homo sapiens, human |
Tissue: | Colon |
Disease: | Dukes' type B, colorectal adenocarcinoma |
Morphology: | Endothelial-like adherent cells, mostly bipolar with microvilli growing in monolayers |
Growth Properties: | Adherent |
III. Representative Data | |
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Figure 1. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System.. | |
IV. Stablity Test | |
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Figure 2. Stablity test results. SW480-Luc cells showed at least 32 passage stability with similar luminescence unit. |