K562-Luc

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RQP30133L
I. Introduction
Host Cell:K562 (BCR-ABL1 fusion)
Expressed gene:Luciferase
Stability:32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium:90% FBS+10% DMSO
Culture Medium:RPMI-1640+10%FBS+1ug/ml puromycin
Mycoplasma Status:Negative
Storage:Liquid nitrogen
 
II. Description of Host Cell Line
Organism:Homo sapiens, human
Tissue:Bone marrow
Disease: Chronic myelogenous leukemia (CML)
Morphology: Lymphoblast
Growth Properties: Suspension
 
III. Representative Data

Figure 1. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System..
The number of Luminescence signal i 584±33(Mean±SD) on K562 cells while 158031±6492(Mean±SD) on K562 -Luc cells.

 
IV. Stablity Test

Figure 2. Stablity test results. K562 -Luc cells showed at least 32 passage stability with similar luminescence unit.

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