4T1-Luc

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RQP30102L
I. Introduction
Host Cell: 4T1 (ATCC #CRL-2539)
Expressed gene: Luciferase
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium: 90% FBS+10% DMSO
Culture Medium:RPMI-1640+10%FBS+2ug/ml puromycin
Mycoplasma Status: Negative
Storage: Liquid nitrogen

II. Description of Host Cell Line
Organism: Mus musculus, mouse
Tissue: Mammary gland
Disease: An animal stage IV human breast cancer.
Morphology: Epithelial
Growth Properties: Adherent

III. Representative Data

Figure. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System.
The number of Luminescence signal is 775±49(Mean±SD) on 4T1 cells while 609590±12940(Mean±SD) on 4T1-Luc cells.

 
IV. Stablity Test

Figure.Stablity test results. 4T1-Luc cells showed at least 32 passage stability with similar luminescence unit. 


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