| RQP30197L | |
| I. Introduction | |
| Host Cell: | HCC1569 (HER2 positive, ER and PR negative) |
| Expressed gene: | Luciferase |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+1ug/ml puromycin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen |
| II. Description of Host Cell Line | |
| Organism: | Homo sapiens, human |
| Tissue: | Mammary gland? breast |
| Disease: | TNM stage IV, grade 3, primary metaplastic carcinoma |
| Morphology: | Epithelial |
| Growth Properties: | Mixed adherentsuspension |
| III. Representative Data | |
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Figure. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. | |
| IV. Stablity Test | |
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Figure.Stablity test results. HCC1569-Luc cells showed at least 32 passage stability with similar luminescence unit. | |
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