HepG2-Luc

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RQP30043L
I. Introduction
Host Cell: HepG2
Expressed gene: Luciferase
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium: 90% FBS+10% DMSO
Culture Medium:MEM + 10% FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)+2ug/ml puromycin
Mycoplasma Status: Negative
Storage: Liquid nitrogen

II. Description of Host Cell Line
Organism: Homo sapiens, human
Tissue: Liver
Disease: Hepatocellular carcinoma
Morphology: Epithelial
Growth Properties: Adherent
 
III. Representative Data

Figure. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System..
The number of Luminescence signal is 464±17(Mean±SD) on HepG2 cells while 178815±8082 (Mean±SD) on HepG2-Luc cells.


IV. Stablity Test

Figure.Stablity test results. HepG2-Luc cells showed at least 32 passage stability with similar luminescence unit.


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