Huh7-Luc

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RQP30045L
I. Introduction
Host Cell: HUH-7
Expressed gene: Luciferase
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium:

90% Complete Medium+10%DMSO

Culture Medium: DMEM+10%FBS+2ug/ml puromycin
Mycoplasma Status: Negative
Storage: Liquid nitrogen

II. Description of Host Cell Line
Organism: Homo sapiens, human
Tissue:

Liver, gallbladder

Disease: Well differentiated hepato cellular carcinoma.
Morphology:

Epithelial-like

Growth Properties: Adherent

III. Representative Data

Figure. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System..
The number of Luminescence signal is 512±8(Mean±SD) on HUH-7 cells while 483768±15940(Mean±SD) on HUH-7-Luc cells.

 
IV. Stablity Test

Figure. Stablity test results.HUH-7-Luc cells showed at least 32 passage stability with similar luminescence unit.


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