K562-Luc

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RQP30133L
I. Introduction
Host Cell: K562 (BCR-ABL1 fusion)
Expressed gene: Luciferase
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: RPMI-1640+10%FBS+2ug/ml puromycin
Mycoplasma Status: Negative
Storage: Liquid nitrogen

II. Description of Host Cell Line
Organism: Homo sapiens, human
Tissue: Bone marrow
Disease: Chronic myelogenous leukemia (CML)
Morphology: Lymphoblast
Growth Properties: Suspension
 
III. Representative Data

Figure. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System..
The number of Luminescence signal i 584±33(Mean±SD) on K562 cells while 158031±6492(Mean±SD) on K562 -Luc cells.


IV. Stablity Test

Figure.Stablity test results. K562 -Luc cells showed at least 32 passage stability with similar luminescence unit.


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