LNCAP-Luc

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RQP30195L
I. Introduction
Host Cell: LNCAP (AR positive, p.T868A)
Expressed gene: Luciferase
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: RPMI-1640+15%FBS+1ug/ml puromycin
Mycoplasma Status: Negative
Storage: Liquid nitrogen

II. Description of Host Cell Line
Organism: Homo sapiens, human
Tissue: Prostate
Disease: Prostate carcinoma
Morphology: Adherent fibroblastoid cells growing in aggregates and as single cells
Growth Properties: Mixed adherentsuspension
 
III. Representative Data

Figure. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System.
The number of Luminescence signal is 445±18(Mean±SD) on LNCAP cells while 104579±1558(Mean±SD) on LNCAP-Luc cells.


IV. Stablity Test

Figure.Stablity test results. LNCAP-Luc cells showed at least 32 passage stability with similar luminescence unit. 


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