LS174T-Luc

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RQP30010L
I. Introduction
Host Cell: LS 174T
Expressed gene: Luciferase
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: 1640+10%FBS+2ug/ml puromycin
Mycoplasma Status: Negative
Storage: Liquid nitrogen

II. Description of Host Cell Line
Organism: Homo sapiens, human
Tissue: Colon
Disease: Dukes' type B,colorectal adenocarcinoma
Morphology: Epitheloid cell
Growth Properties: Adherent

III. Representative Data

Figure. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System.
The number of Luminescence signal is 503±12(Mean±SD) on LS174T cells while 58832±2614(Mean±SD) on LS174T-Luc cells.

 
IV. Stablity Test

Figure. Stablity test results. LS174T-Luc cells showed at least 32 passage stability with similar luminescence unit.


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