MBT-2-Luc

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RQP30171L
I. Introduction
Host Cell: MBT-2
Expressed gene: Luciferase
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: MEM + 10% FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)+1ug/ml puromycin
Mycoplasma Status: Negative
Storage: Liquid nitrogen

II. Description of Host Cell Line
Organism:Mus musculus, mouse
Tissue: Bladder
Disease: bladder transition, mouse
Morphology: Epithelial-like
Growth Properties: Adherent

III. Representative Data

Figure. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System..
The number of Luminescence signal is 533±8(Mean±SD) onMBT-2cells while 714180±26582(Mean±SD) onMBT-2-Luc cells.


IV. Stablity Test

Figure. Stablity test results.MBT-2-Luc cells showed at least 32 passage stability with similar luminescence unit.


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