NCI-H1299

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RQP30016L
I. Introduction
Host Cell:NCI-H1299
Expressed gene:Luciferase
Stability:32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium:90% FBS+10% DMSO
Culture Medium:RPMI-1640 Medium + 10% FBS+2 ug/ml puromycin
Mycoplasma Status:Negative
Storage:Liquid nitrogen

II. Description of Host Cell Line
Organism:Homo sapiens, human
Tissue:Lung; derived from metastatic site: lymph node
Disease: Carcinoma; non-small cell lung cancer
Morphology: Epithelial
Growth Properties: Adherent

III. Representative Data

Figure. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System..
The number of Luminescence signal is 510±15(Mean±SD) on NCI-H1299 cells while 113348149 ±3416839(Mean±SD) on NCI-H1299-Luc cells.


IV. Stablity Test

Figure. Stablity test results.NCI-H1299-Luc cells showed at least 32 passage stability with similar luminescence unit.


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