NCI-H1975-Luc

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RQP30029L
I. Introduction
Host Cell: NCI-H1975 (EGFR p.L858R/T790M)
Expressed gene: Luciferase
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium: 90% FBS+10% DMSO
Culture Medium:RPMI-1640+10%FBS+2ug/ml puromycin
Mycoplasma Status: Negative
Storage: Liquid nitrogen

II. Description of Host Cell Line
Organism: Homo sapiens, human
Tissue: Lung
Disease: Adenocarcinoma; non-small cell lung cancer
Morphology: Epithelial
Growth Properties: Adherent

III. Representative Data

Figure. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System..
The number of Luminescence signal is 549±33(Mean±SD) on NCI-H1975 cells while 641009±23361(Mean±SD) on NCI-H1975-Luc cells.

 
IV. Stablity Test

Figure. Stablity test results. NCI-H1975-Luc cells showed at least 32 passage stability with similar luminescence unit.


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