| RQP30208G | |
| I. Introduction | |
| Host Cell: | NCI-H2122 |
| Expressed gene: | Green Fluorescent Protein |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640 Medium + 10%FBS+1 ug/ml puromycin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen |
| II. Description of Host Cell Line | |
| Organism: | Homo sapiens, human |
| Tissue: | Lung; derived from metastatic site: pleural effusion |
| Disease: | Stage 4, adenocarcinoma; non-small cell lung cancer |
| Morphology: | It is normal for CRL-5985 to show a mixed morphology of rounded cells and epithelial cells. This cell line produces moderate to large, viable, loose, grape-like clusters that either loosely adhere to the flask or remain in suspension |
| Growth Properties: | Adherent |
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