NCI-H460-Luc

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RQP30033L
I. Introduction
Host Cell: NCI-H460
Expressed gene: Luciferase
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: RPMI-1640+10%FBS+2ug/ml puromycin
Mycoplasma Status: Negative
Storage: Liquid nitrogen

II. Description of Host Cell Line
Organism: Homo sapiens, human
Tissue: Lung: pleural effusion
Disease: Carcinoma; large cell lung cancer
Morphology: Epitheloid cell
Growth Properties: Mixed adherentsuspension

III. Representative Data

Figure. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System..
The number of Luminescence signal is 475±15(Mean±SD) on NCI-H460 cells while 902713±23711(Mean±SD) on NCI-H460-Luc cells.


IV. Stablity Test

Figure.Stablity test results. NCI-H460-Luc cells showed at least 32 passage stability with similar luminescence unit.


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