| RQP73388 | |
| I. Introduction | |
| Cell Line Name： | BRAF K601E/BaF3 |
| Host Cell: | Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Application: | Anti-proliferation assay and PD assay |
| Freeze Medium: | 90% FBS+10% DMSO |
| Complete Culture Medium: | RPMI-1640+10%FBS+2ug/ml puromycin |
| Mycoplasma Status: | Negative |
| II.Background | |
| BRAF K601E lies within the activation segment in the kinase domain of the Braf protein. K601E results in increased Braf kinase activity and downstream activation of MEK and ERK in cell culture, and induces cell proliferation and cell viability in culture. | |
| III. Representative Data | |
1. WB of BRAF K601E/BaF3
2. Sanger of BRAF K601E/BaF3
Figure 3. Sanger of BRAF K601E/BaF3 | |
3. Anti-proliferation assay | |
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Figure 3. CTG Proliferation Assay of BRAF K601E BaF3 Cells (C1). | |
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