| RQP73387 | |
| I. Introduction | |
| Cell Line Name： | BRAF L597R/BaF3 |
| Host Cell: | Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Application: | Anti-proliferation assay and PD assay |
| Freeze Medium: | 90% FBS+10% DMSO |
| Complete Culture Medium: | RPMI-1640+10%FBS+2ug/ml puromycin |
| Mycoplasma Status: | Negative |
| II.Background | |
| BRAF L597R lies within the protein kinase domain of the Braf protein (UniProt.org). L597R results in activation of Braf as indicated by increased phosphorylation of Mek and Erk in cell culture, is associated with Erk activation in a patient tumor sample, and in one of two cell lines, increased cell proliferation and cell viability compared to wild-type Braf. | |
| III. Representative Data | |
1. WB of BRAF L597R/BaF3
2. Sanger of BRAF L597R/BaF3
Figure 2. Sanger of BRAF L597R/BaF3 | |
3. Anti-proliferation assay | |
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Figure 3. CTG Proliferation Assay of BRAF L597R BaF3 Cells (C3). | |
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