| RQP73385 | |
| I. Introduction | |
| Cell Line Name： | BRAF V600R/BaF3 |
| Host Cell: | Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Application: | Anti-proliferation assay and PD assay |
| Freeze Medium: | 90% FBS+10% DMSO |
| Complete Culture Medium: | RPMI-1640+10%FBS+2ug/ml puromycin |
| Mycoplasma Status: | Negative |
| II.Background | |
| BRAF V600R (previously reported as V599) lies within the activation segment of the kinase domain of the Braf protein. V600R confers a gain of function to the Braf protein as demonstrated by increased Braf kinase activity, downstream signaling, and the ability to transform cells in culture. | |
| III. Representative Data | |
1. WB of BRAF V600R/BaF3
2.Sanger of BRAF V600R/BaF3
Figure 2. Sanger of BRAF V600R/BaF3 | |
3. Anti-proliferation assay | |
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Figure 3. CTG Proliferation Assay of B-RAF V600R BaF3 Cells (C14). | |
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