Detailed introduction

RQP73212
I. Introduction
Cell Line Name：	EGFR DEL19-T790M-C797S/PC9
Host Cell:	PC9
Stability:	16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Application:	Anti-proliferation assay and Cell-based kinase assay
Freeze Medium:	90% FBS+10% DMSO
Complete Culture Medium:	RPMI-1640+10%FBS+2ug/ml puromycin
Mycoplasma Status:	Negative

II.Background
EGFR is widely recognized for its importance in cancer. Amplification and mutations have been shown to be driving events in many cancer types. Its role in non-small cell lung cancer, glioblastoma and basal-like breast cancers has spurred many research and drug development efforts. Tyrosine kinase inhibitors have shown efficacy in EGFR amplfied tumors, most notably gefitinib and erlotinib. Mutations in EGFR have been shown to confer resistance to these drugs, particularly the variant T790M, which has been functionally characterized as a resistance marker for both of these drugs. The later generation TKI's have seen some success in treating these resistant cases, and targeted sequencing of the EGFR locus has become a common practice in treatment of non-small cell lung cancer. Overproduction of ligands is another possible mechanism of activation of EGFR. ERBB ligands include EGF, TGF-a, AREG, EPG, BTC, HB-EGF, EPR and NRG1-4 (for detailed information please refer to the respective ligand section). In ligand-activated cancers, Cetuximab appears to be more effective than tyrosine-kinase inhibitors.

III. Representative Data
1.WB of EGFR DEL19-T790M-C797S/PC9 expression
2.Sanger of EGFR DEL19-T790M-C797S/PC9 Figure 2. EGFR DEL19-T790M-C797S/PC9 19del Figure 3. EGFR DEL19-T790M-C797S/PC9 T790M Figure 4. EGFR DEL19-T790M-C797S/PC9 C797S
3.Anti-proliferation assay

Figure 5. Anti-proliferation assay of three reference compounds on theCCK8 Proliferation Assay of EGFR DEL19-T790M-C797S/PC9 cells Line.