| RQP71423 | |
| I. Background | |
| The glucagon-like peptides include glucagon, GLP-1, and GLP-2 exert diverse actions on nutrient intake, gastrointestinal motility, islet hormone secretion, cell proliferation and apoptosis, as well as nutrient absorption and assimilation. Glucagon-like peptide (GLP)-2, a product of the proglucagon gene, is expressed in enteroendocrine cells of the small and large intestine and is trophic to the gastrointestinal mucosa. GLP-2 also inhibits gastric acid secretion and emptying and up-regulates intestinal hexose transport. GLP-2 acts via binding to a single G protein-coupled GLP-2 receptor (GLP-2R). | |
| II. Introduction | |
| Host Cell: | CHO |
| Stability: | 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12k+10%FBS+5ug/ml puromycin+5ug/ml blasticidin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): | Functional assay for GLP2R receptor |
| Assay Format: | β-arrestin recruitment |
| Ⅲ. Description of Host Cell Line | |
| Organism: | Hamster |
| Tissue: | Ovary |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| Ⅳ. Representative Data | |
Figure 1. Recombinant GLP2R/β-Arrestin/CHO constitutively expressing GLP2R.
Figure 2. Dose response of Human GLP-2(1-33) in GLP2R β-Arrestin CHO-K1 Cell Line (C5). | |
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